LC/MS/MS

Liquid chromatography–Mass spectrometry (LC/MS, or HPLC/MS) Is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry. LC/MS is a powerful technique used for many applications which has very high sensitivity and selectivity. Generally its application is oriented towards the specific detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture).

AmeriDrug Labs uses a multiple of “triple quadrupole mass spectrometer” which is a tandem mass spectrometer consisting of two quadrupole mass spectrometers in series, with a (non mass-resolving) radio frequency (RF) only quadrupole between them to act as a collision cell for collision-induced dissociation. The first (Q1) and third (Q3) quadrupoles serve as mass filters, whereas the middle (q2) quadrupole serves as a collision cell. This collision cell is an RF only quadrupole (non-mass filtering) using an inert gas such as Ar, He, or N2 gas to provide collision-induced dissociation of a selected precursor ion that is selected in Q1. Subsequent fragments are passed through to Q3 where they may be filtered or scanned. This configuration is often abbreviated QqQ, here Q1q2Q3.